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viernes, 13 de agosto de 2010


Practical Genetics
European Journal of Human Genetics (2010) 18, 265–271;
Triple X syndrome: a review of the literature
Maarten Otter1,2, Constance TRM Schrander-Stumpel3 and Leopold MG Curfs3
1Department of Child and Adolescent Psychiatry, Eleos, Amersfoort, The Netherlands
2Department of Adult Psychiatry, Eleos, Amersfoort, The Netherlands
3Department of Clinical Genetics, Academic Hospital Maastricht, GROW-School for Oncology and Developmental Biology, University Maastricht, Maastricht, The Netherlands
Correspondence: Dr M Otter, Department of Child and Adolescent Psychiatry, Department of Adult Psychiatry, Printerweg 21/23, Amersfoort 3821 AP, The Netherlands. Tel: +31 33 45 71 717; Fax: +31 318 67 29 41; E-mail:
The developmental and clinical aspects in the literature on triple X syndrome are reviewed. Prenatal diagnosis depends on karyotyping. The incidence is 1 of 1000 females. At birth, 47,XXX girls have a lower mean birth weight and a smaller head circumference. Triple X diagnosis was not suspected at birth. The maternal age seems to be increased. Toddlers with triple X syndrome show delayed language development. The youngest girls show accelerated growth until puberty. EEG abnormalities seem to be rather common. Many girls show motor-coordination problems and auditory-processing disorders are not rare. Scoliosis is probably more common in adolescent cases. The IQ levels are 20 points below that of controls, and verbal IQ is lowest. The girls struggle with low self-esteem and they need psychological, behavioural and educational support. They perform best in stable families. After leaving school they seem to feel better. In adults, premature ovarian failure seems to be more prevalent than in controls. MRIs of the brain seem to show decreased brain volumes. The 47,XXX women most often find jobs that reflect their performance abilities. Psychotic illness seems to be more prevalent in triple X adult women than in controls. Psychotic disorders respond well to psychotropic drugs. Triple X adults suffer more frequently from cyclothymic and labile personality traits. Research on triple X syndrome may yield more insight into brain and behaviour relations, developmental psychopathology, auditory-processing disorders, EEG disorders, personality and psychotic disorders, etc.

Keywords: review, medical genetics, sex-chromosome aberrations, behavioural phenotypes of genetic syndromes, development, psychiatric disorders

In brief
•One in every 1000 females has an extra X chromosome.
•The physical phenotype shows earlier growth and longer legs.
•The behavioural phenotype often shows auditory processing disorders, disorders in language development and problems in forming stable interpersonal relationships.
•Psychiatric disorders seem to be more common in triple X syndrome.
•Quality of life seems to increase after leaving school.
•Further research is necessary. This is true in physical and behavioural aspects.
Top of pageIntroduction
Triple X syndrome (47,XXX) is not extremely rare, although one might think so, as the majority of cases go undiagnosed.1 The incidence has been established to 1/1000 females, since the earliest case series have been published and confirmed by others.2 Triple X syndrome is a sex chromosomal abnormality (SCA). The sex chromosomes have aneuploid counts in SCA, e.g. 47,XYY, 47,XXY, 47,XO, etc. SCA may be mosaic.3

After the description of Down's anomaly as trisomy 21,4 Klinefelter syndrome as 47,XXY5 and Turner's syndrome as 45,XO,6 the first triple X case was published.7 In 1959, Jacobs described the first 47,XXX female, an infertile patient. A similar condition had already been described in infertile Drosophila melanogaster.7 In contrast to Down's syndrome, Klinefelter syndrome and Turner's syndrome, there were no clinical descriptions of triple X syndrome before the technique of karyotyping became available.8, 9, 10, 11

In cases of prenatal diagnosis, parents and clinical genetic counsellors needed information about this syndrome. The review by Nielsen has been worthwhile;12 this information is freely available on the internet and has been translated into several languages by parent support groups (see for instance: After postnatal diagnosis, this information is useful for parents and for clinical geneticists, paediatricians, gynaecologists, (child) neurologists, (child) psychiatrists and others. This article aims to provide another up-to-date review of the literature.

After having described the literature search method, we will present literature findings on physical and psychological/behavioural issues. We will then discuss the findings taken from several age groups and offer some suggestions for future research.

Materials and methods
Reference bias is a major confounder in the literature on triple X syndrome. In studies with a high level of reference bias, one might get the impression that triple X syndrome is associated with a high level of malformations and pathology.13, 14 This is in contrast with studies with low levels of reference bias.

The lowest level of bias in the field of SCA is found in newborn screening studies, initiated by the March of Dimes Birth Defects Foundation. We will refer to them in this paper as ‘unbiased studies’ or ‘newborn-screening studies’ in the description of development in the consecutive age groups. The March of Dimes Birth Defects Foundation organized a conference in Aspen in 1974. The visiting researchers decided to screen almost 200 000 newborns from several cities (Aarhus, Denmark; Toronto, Canada; New Haven, Connecticut, USA; Denver, Colorado, USA; Edinburgh, Scotland and Winnipeg, Canada) for SCA. The cases with SCA were investigated in a prospective study design for 20 years or, in the cases from Edinburgh and Denver, even longer. These researchers met four times from 1979 until 1989. The conference proceedings were published in four issues of the Birth Defect Original Article Series,15, 16, 17, 18 and each editor published a summary of the results.19, 20, 21, 22 These issues give detailed information on the development of triple X syndrome (and other SCA) collected by multi-disciplinary teams consisting of clinical cytogeneticists, paediatricians, psychologists, psychiatrists, social workers and speech and language therapists. No (child) neurologists, endocrinologists or ophthalmologists were involved.

We carried out a PubMed search using the following search terms: ‘superfemale’, ‘sex chromosomal disorder’, ‘sex chromosomal anomaly’, ‘sex chromosome aneuploidy’, ‘triple X (syndrome)’, ‘triplo X (syndrome)’ and ‘47,XXX’. These studies are more or less biased because of referral bias. We scrutinized references for every paper, book, etc. This was necessary to find papers from the Oldmedline database,13, 23, 24 chapters of books25 and theses,26, 27, 28 etc.29

When referring to reviews, we did not refer to any references mentioned therein to keep this paper as concise as possible. We excluded case reports on single cases, as it is not clear whether the level of incidence goes beyond pure coincidence. We focused on studies with clinical information and excluded others, e.g. papers focusing exclusively on molecular biology.

Development and clinical features in triple X syndrome
To describe development in triple X syndrome in relation to age, we arranged the available data in consecutive age groups, starting with the prenatal cases. In each postnatal age group, we described physical development based on the newborn screening, unbiased, studies and added data from biased studies; psychological and behavioural development are subsequently described in a similar way (Table 1).

Prenatal diagnosis
To the best of our knowledge, prenatal cases of triple X syndrome have not been suspected on just clinical grounds. The association between trisomy X in the offspring and maternal age is not as strong as in trisomy 21.29 In the newborn-screening studies, the average maternal age was 33 and the paternal age was 29.19

In cases of SCA in general, counsellors have questioned the reliability of the available data on these disorders.33, 34 Many data were collected from biased case series as we mentioned above. Data from unbiased studies on development until adulthood became available in 1990,22 almost 15 year after the first reports on genetic counselling in SCA.35, 36

47,XXX neonates: are there congenital malformations?
In the first papers on the newborn-screening studies, 43 girls with an extra X chromosome were described.19 The prevalence seemed to be 0.1%.2 The mean birth weight was 2979 g, 400–500 g less than that in the controls. The aneuploid status would not have been suspected at birth. Only clinodactyly and epicanthal folds were seen as minor anomalies in some of the cases. Two cases were reported with congenital heart disease, one being a patent ductus arteriosus.19, 37

The biased studies more often show malformations. Ten years after the first description of triple X syndrome, one-third of 155 cases had a congenital physical defect.13 In his thesis on 39 women with an extra X chromosome, Olanders38 reported non-specific facial dysmorphism (mandibular prognathism as well as retrognathism) and various minor hand and feet anomalies, with clinodactyly or short fifth finger being a rather frequent finding. In 8 of 39 cases, parents were available for a detailed history. No one suspected the aneuploid status of the girls when they were born.39 Guichet14 reported 2 of 190 cases with clubfoot (pes equinovarus). Heart anomalies are rare in triple X syndrome;14, 40 the numbers do not exceed the population prevalence numbers on heart anomalies, this being 0.8%.41 Cases with strabismus have been described.39, 42 Haverty43 described 16 patients, including 13 previously reported patients, with genitourinary malformations (Table 2).
Youngest 47,XXX girls
The newborn-screening studies on early development contained data on the development of 49 girls. Their height seemed to be normal before they reached the age of four. Later, they seem to be somewhat taller, with 17 out of 20 girls being taller than the 50th percentile. Their legs are longer than expected for their heights. The head circumferences were smaller, with 19 of 25 of them below the 50th percentile.19
Seven cases from Aarhus, Denmark showed a significantly higher interpupillary distance.55 Twelve cases from Toronto showed FSH levels as low as girls with gonadal dysgenesis (eg Turner's syndrome).56 Eleven girls from Denver started to walk later than the controls (11–22 months) and showed coordination problems.57
The full-scale IQ's were significantly lower than in controls.19 Language development (receptive and expressive language) was delayed in 19 out of 41 girls. Methodological problems made it difficult to make comparisons between the data from the participating research groups. More cases than controls showed shyness, un-cooperativeness, clumsiness and attention deficits.55 Two of the twelve girls from Toronto were referred for child psychiatric examination because of temper tantrums and resistive behaviour.56 Four of the eleven cases from Denver required psychiatric intervention because of externalizing or internalizing psychiatric disorders. There were signs of parental marital distress in 6 of 11 cases, higher than in other SCA syndromes.57 Three of the eleven cases from Edinburgh showed behavioural problems, one due to hyperactivity, one probably attributed to epilepsy and the other to shyness.37

47,XXX girls at school
Data on physical development from 6–13 years of age show 16 of 38 (42.1%) 47,XXX girls grew >90th percentile and 9 of 38 (23.7%) grew <50th p="0.059)," p="0.038)," p="0.040)," p="0.003)," p="0.038)" p="0.00001)." cases="90.06" controls="108.40)," cases="86.63" controls="105.64)" cases="95.16" controls="109.60),">25 years of age) proved to be fertile.39 After the first papers on fertile 47,XXX women,78, 79 fertility no longer seemed to be an issue.
As far as we know, there is just one report on a 47,XXX woman with a 47,XXX offspring.43 Premature ovarian failure probably does exceed the population risk 45, 46, 80, 48 (Table 2).
The newborn-screening studies did not involve EEG recordings. One case of epilepsy was reported. This girl was reported to be extremely shy and had frequent temper tantrums. The details about the therapy have not been published.37 Olanders49 reported in his case series some form of paroxysmal abnormality in 13/21 cases, in four cases after photic stimulation. A common finding was a low-frequency background. His literature review showed that paroxysmal activity or increased photosensitivity occurred in 13/48 cases. These results were comparable with those found in Klinefelter syndrome.81 Other authors suggested that EEG anomalies in triple X syndrome are associated with mental retardation. Treatment with carbamazepine was effective in all but one case50, 51 (Table 2).
IQ scores in the Edinburgh group showed data similar to earlier findings in the same cases. Common findings in psychiatric interviews consisted of slow psychic tempo, fatigability, under-initiative in making social contact, limited ability in verbal and non-verbal language and conversation skills, an anxious affective display, an asthenic appearance and low self-esteem, with low self-esteem being the most common finding. Four of the sixteen triple X girls (in controls 2 of 75) were referred to a psychiatrist: one case of trichotillomania and three cases of behavioural disorders. Two of these four patients were diagnosed with a major depressive disorder. Two cases were diagnosed as cyclothymic personality and three for labile personality. One case suffered from non-psychotic ideas of reference and dissociative periods.82
Warwick83 re-examined these cases three years later and found increased social anxiety, suspiciousness, restricted emotion and impulsivity/non-conformity. Antisocial and schizotypical traits were rare but more prevalent in cases than in controls. The same was true for irritability and anger. These were associated with lower scores on IQ testing. There seemed to be some improvement in quality of life after leaving school.
The Denver group described the occurrence of learning disabilities, language disorders and motor disorders in young adults: ‘The (unselected) 47,XXX women during adolescence and young adulthood were less well adapted; had more stress; had more work, leisure and relationship problems; had a lower IQ; and showed more psychopathology when contrasted with the comparison group.84 Eight of the eleven 47,XXX females showed neurocognitive and psychosocial impairment.74, 85, 30, 31 They carried out follow-up examinations on these girls and other cases, which had been diagnosed prenatally. Apparently, the prenatally diagnosed were of a higher socio-economic status, predicting a higher level of functioning.32
Psychotic disorders have been described in 47,XXX syndrome (Table 2).52, 53, 86 Olanders39 described 20 adult cases recruited from mental hospitals; in these cases, the psychiatric illness started between 22 and 73 years of age. Four of these women required an inpatient treatment, as they were under the age of 32. In general, psychotic symptoms reacted well to standard neuroleptic treatment. Olanders39 reported effective treatment in four cases using electro-convulsive therapy. No findings were reported on the treatment of psychiatric symptoms using anti-epileptic drugs.49

To the best of our knowledge, no data are available on old age development in triple X syndrome.

Although there seems to be a general consensus on providing genetic information in a nondirective manner,87 parents have received conflicting information after a prenatal SCA diagnosis.88, 89 After a prenatal triple X diagnosis, a lower pregnancy termination rate is reported than in Klinefelter syndrome.90, 91 Severe abnormalities in ultrasound examinations before or after prenatal diagnosis increase termination rates.92 There are major cultural differences in prenatal counselling.93, 94 For instance, in Israel, high rates of termination are reported,95 but low rates are reported in Germany.96, 97 The reports on development in unbiased groups with SCA seem to be responsible for a trend of a decline in termination rates.98, 99, 100, 101
In neonatal cases with malformations, there are no reports on prevalence numbers that exceed the population risk. The only exception may be genitourinary malformations (Table 2). In cases with a prenatal 47,XXX diagnosis, it seems to be common sense to give special attention to the urogenital tract in prenatal ultrasound investigations to offer the opportunity of early intervention after birth.
The youngest cases seem to suffer from a delay in language development. Auditory-processing disorders may be responsible for this delay. Furthermore, delay in language development may be responsible for a delay in social cognition and functioning.102 This might explain some of the problems in forming stable interpersonal relationships.
Clear endocrine disorders have been found in adolescent Klinefelter boys and testosterone therapy has been accepted.103 In triple X syndrome, endocrinological findings from the newborn-screening study are not available. This may have consequences for the psychological development. Estrogen functions as a neuroprotective agent so, if women lack sufficient estrogen levels, this might (partially) explain behavioural disorders.104, 105, 106, 107
Several studies have described psychiatric disorders in adults. It may be difficult to make a comparison with modern studies because these studies did not use the currently accepted classification according to the DSM IV-TR.108 Moreover, it is necessary to consider that cases from the newborn-screening studies have not yet been examined in their thirties, an age at which vulnerability for psychiatric disorders is expected to become clinically recognizable.
Most of the published triple X patients with psychotic disorders show paranoid symptoms.52 Low self-esteem has been reported in almost all the young adult women in the Edinburgh newborn-screening study.82 Psychological studies have shown an association between low and unstable self-esteem in paranoid patients.109, 110 Further research is necessary to know whether this may (partially) explain the prevalence of paranoid symptoms in psychotic triple X women. Some authors argue that psychosis in cytogenetic disorders might shed some light on the aetiology of psychosis and we agree.111, 112
Although developmental biology is not the focus of this review, further research in this field may yield a better understanding and findings may be clinically relevant. We want to highlight three issues: the concept regarding the slowing down of cell cycles in trisomic cells as an explanation of the phenotype in developmental disorders, the influence of the slowing down of the cell cycles on foetal and adult neurogenesis and the question of whether incomplete X-chromosomal inactivation in trisomy X might negatively influence brain development.
So first, Polani (1977) and others have discussed decreased cell-division rates in SCA syndromes as a probable explanation of the phenotype as reflected in dermatoglyphics113 and reduced brain volumes114, 115 in SCA.25, 116 The cell cycle slows down in cases with an extra X chromosome117 and in other cells with an extra chromosome in humans117, 118 as well as in yeast119 and in seedlings.120 DNA replication is apparently the rate-limiting step in the cell cycle.120
Secondly, there is a growing body of literature on the association between psychiatric diseases and adult neurogenesis.121, 122, 123, 124 The dysfunction of DISC1 (disrupted-in-schizophrenia-1) disrupts adult neurogenesis and causes a variety of psychotic and affective disorders.125 Adult neurogenesis is relevant to the function of the amygdala, hippocampus, the olfactory region and the substantia nigra.126, 127 A slowing of the cell-division rate might—as in prenatal development of the brain—influence the efficacy of adult neurogenesis. Further research in triple X syndrome might throw more light on the aetiology of psychotic and affective disorders through delayed adult neurogenesis.
Thirdly, without the inactivation of the second X chromosome, women would not survive (for a review see Migeon128). A recent study has shown that this inactivation is incomplete. Fifteen percent of the X chromosomal genes are just partially inactivated or not inactivated.129, 130, 131 In men with an extra X chromosome, the second X chromosome is inactivated. However, RNA studies in Klinefelter have proven that there are differences in RNA levels between XY and XXY men.132 The third X chromosome in 47,XXX women seems to be inactivated.133 However, it seems to be reasonable to hypothesize that in XXX females, RNA levels differ from XX controls. The X chromosomal genetic disorders are often related to mental retardation. Many X chromosomal genes must be involved in brain development.134 So an unbalanced expression of X chromosomal genes may contribute to sub-optimal brain development. The neuropathological studies will be necessary to throw more light on typical and atypical brain development in trisomy syndromes.
Until now, these three scientific issues have almost not been studied in humans or in animal models, although animal models are available, especially in infertile cattle.135, 136, 137, 138
Preventive managment
Preventive management is meant to prevent developmental and educational delay. As we mentioned above, EEG abnormalities, urogenital disorders and ophthalmological disorders seem to be more common in triple X syndrome and require clinical attention (Table 2). We recommend regular neuropsychological and linguistic testing using test batteries suited to the consecutive age groups. Our threshold for a thorough assessment is even lower than that recommended by others.139 It is our clinical experience that developmental, behavioural or educational difficulties are often caused by linguistic, auditory and neurocognitive disorders as well as a lack of awareness and understanding on the part of clinicians, parents, teachers, etc. (Table 3).
Triple X syndrome is a syndrome with a high level of variety in the physical and behavioural phenotype. Triple X syndrome is not rare, but it is often undiagnosed. Notwithstanding the relatively high prevalence of triple X syndrome, there are many issues yet to be studied in physical and behavioural development up to old age.
First of all, it would be interesting to continue the follow-up of the girls from the unbiased longitudinal studies and start new cohorts of longitudinal studies. In psychiatric research, it would be interesting to study the relation between low self-esteem and paranoid ideations. In addition, the relations between auditory-processing disorders, language development and the development of social cognition may yield insight into the development of difficulties in forming satisfying relationships and how to treat disorders in this area.
Triple X syndrome may serve as a model for studying the physical and behavioural aspects of developmental delay. The process of X chromosome inactivation in trisomy X and other scientific issues require further study. In this field, insight into the pathology may yield more insight into the physiology.
Above all, further study is needed to establish evidence-based treatment and support protocols in physical treatments (endocrinological treatment, fertility issues and treatment in cases with EEG anomalies in relation to behaviour, etc.), educational support, psychiatric diagnosis and treatment, and psychological treatment, such as psychotherapy and family therapy.
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3.Robinson A, Linden MG, Bender BG: Prenatal diagnosis of sex chromosomal abnormalities. In: Milunsky A (ed): Genetic Disorders and the Fetus; Diagnosis, Prevention and Treatment, 4th edn Baltimore and London: Johns Hopkins, 1998, pp 249–285.
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